anti atg13 Search Results


atg13  (Bioss)
94
Bioss atg13
Atg13, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atg13  (GeneTex)
90
GeneTex atg13
Atg13, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibodies against atg12  (MBL Life science)
90
MBL Life science monoclonal antibodies against atg12
(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound <t>ATG5-ATG12.</t> Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.
Monoclonal Antibodies Against Atg12, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
monoclonal antibodies against atg12 - by Bioz Stars, 2026-03
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anti-atg13  (Merck KGaA)
90
Merck KGaA anti-atg13
(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound <t>ATG5-ATG12.</t> Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.
Anti Atg13, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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anti-atg13 m183-3  (MBL International)
90
MBL International anti-atg13 m183-3
(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound <t>ATG5-ATG12.</t> Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.
Anti Atg13 M183 3, supplied by MBL International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti-atg13  (Arigo Biolaboratories)
90
Arigo Biolaboratories rabbit polyclonal anti-atg13
(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound <t>ATG5-ATG12.</t> Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.
Rabbit Polyclonal Anti Atg13, supplied by Arigo Biolaboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti-atg13 - by Bioz Stars, 2026-03
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antigli2 s355 o-glcnac antibody  (ABclonal Biotechnology)
90
ABclonal Biotechnology antigli2 s355 o-glcnac antibody
(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound <t>ATG5-ATG12.</t> Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.
Antigli2 S355 O Glcnac Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal anti-atg13 antibody  (Max Perutz Labs)
90
Max Perutz Labs monoclonal anti-atg13 antibody
(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound <t>ATG5-ATG12.</t> Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.
Monoclonal Anti Atg13 Antibody, supplied by Max Perutz Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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custom antibody anti-phospho-atg13  (Millennium Pharmaceuticals)
90
Millennium Pharmaceuticals custom antibody anti-phospho-atg13
(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound <t>ATG5-ATG12.</t> Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.
Custom Antibody Anti Phospho Atg13, supplied by Millennium Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti atg 13 antibody  (US Biological Life Sciences)
90
US Biological Life Sciences anti atg 13 antibody
(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound <t>ATG5-ATG12.</t> Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.
Anti Atg 13 Antibody, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti atg 13 antibody/product/US Biological Life Sciences
Average 90 stars, based on 1 article reviews
anti atg 13 antibody - by Bioz Stars, 2026-03
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Image Search Results


(A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound ATG5-ATG12. Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.

Journal: PLoS Genetics

Article Title: Reduction of WDR81 impairs autophagic clearance of aggregated proteins and cell viability in neurodegenerative phenotypes

doi: 10.1371/journal.pgen.1009415

Figure Lengend Snippet: (A and B) Number of Htt97Q-EGFP inclusions without (siCtrl) or with knockdown of WDR81 (siWDR81) in HeLa cells stably expressing Htt97Q-EGFP. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 200 cells in 10 fields of each sample were measured. White dotted lines are the outlines of cells. (C and D) Knockdown of WDR81 reduces association between Htt97Q-EGFP and endogenous p62 and LC3-II, but not covalent bound ATG5-ATG12. Representative images of immunoprecipitation of Htt97Q-EGFP and indicated proteins were shown in the upper panel. Statistical analysis of the association between Htt97Q-EGFP and indicated proteins was shown in the bottom panel. (E-I) WDR81-mediated clearance of Htt97Q-EGFP was dependent on lysosome activity. DOX was added to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells. After 12 h, v-ATPase inhibitor, BafA1 (0.4 μM) was added into medium to block capacity of lysosomal degradation. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the bottom panels. (J-M) WDR81-mediated clearance of Htt97Q-EGFP was dependent on ATG5-mediated autophagy. HeLa cells stably expressing Htt97Q-EGFP were transfected twice at an interval of 24 h without or with siATG5. 12 h after the second siRNA transfection, DOX was administrated into medium to induce Htt97Q-EGFP expression. 12 h later, Flag-WDR81 (1.5 μg) was transfected into cells to promote clearance of Htt97Q-EGFP. Statistical analysis of clearance of Htt97Q-EGFP and autophagic proteins was shown in the right panels. Scale bars represent 10 μm in all panels. For quantifications, means±SEM were derived from three independent experiments and analyzed using ANOVA. * P <0.05. ** P <0.01. *** P <0.001. NS, not significant.

Article Snippet: Mouse monoclonal antibodies against ATG12 were purchased from MBL.

Techniques: Knockdown, Stable Transfection, Expressing, Transfection, Immunoprecipitation, Activity Assay, Blocking Assay, Derivative Assay